[Histonet] MART-1/Melan A from LabVision or Neomarkers
I have been trying to use MART-1/Melan-A epitope specific Rabbit Ab on
paraffin sections for flourescence based IHC. However, I see that the
Ab stains quite differently from Pan Melanoma markers or even
monoclonal Mouse Melan-A. I use pressure cooking for 15 min after
boiling the water for my antigen retrieval. I have tried various conc.
of primary Ab and I tried varying the conc. of Alexa F(ab)2 secondary
conjugate. Does anyone have a better experience with this particular
Ab from LabVision or Novocastra?
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