[Histonet] Bouin's alternative

From:Marlene Nardi

We currently use Bouin's solution to fix animal eyes (good retinal 
preservation), to fix animal reproductive tissues (prevents shrinkage of 
testes), and as the post-fix in the Masson Trichrome stain.  Any ideas on a 
less hazardous alternative solution for any of these uses?  For the 
Trichrome stain I have tried the commercial Bouin's substitute, Gram's 
iodine and citrate buffer with limited/no success.
thank you in advance,

Marlene C. Nardi
Technical Services Supervisor
Histology Laboratory
New York State College of Veterinary Medicine
Cornell University
Department of Biomedical Sciences
S2-124 Schurman Hall
Ithaca, NY 14853
ph. 607-253-3314 or 607-253-3303
FAX  607-253-3357

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