FW: [Histonet] OCT und freezing time

From:"Niehans, Gloria"



-----Original Message-----
From: Niehans, Gloria 
Sent: Friday, July 16, 2004 10:21 AM
To: 'Ivo Schmidt'
Subject: RE: [Histonet] OCT und freezing time


It only adds about a minute to the time when the tissue freezes solid.  We
usually left OCT-embedded tissue in the isopentane freezing bath at -80
degrees C for between 5 and 10 minutes, although sometimes when we got busy
the tissue stayed in the freezing bath for up to an hour and it didn't seem
to hurt the tissue at all (isopentane is very inert).  We got good recovery
of RNA and DNA out of tissues handled this way, and tissue architecture was
very good (comparable to frozen sections done in the OR).  

Gloria Niehans

-----Original Message-----
From: Ivo Schmidt [mailto:ivoschi@web.de]
Sent: Friday, July 16, 2004 10:03 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] OCT und freezing time


Hello experts,

I want to freezer my tissue quickly in OCT to preserve
tissue architecture and macromolecules (RNA, DNA, etc)
in isopentane at -80C.
Does anybody know, how much longer you should immerse
an *OCT-embedded tissue* compared to a *naked tissue* (not OCT-embedded) ?

Thanx in advance.

Cordially,

Ivo.


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