FW: [Histonet] Dezenkerizing slides
oops..I forgot to send it to the HistoNet.
From: Carole Fields
Sent: Monday, July 12, 2004 2:55 PM
To: 'Gayle Callis'
Subject: RE: [Histonet] Dezenkerizing slides
It is in Ann Preese, us old-timers remember. 0.5% Alc-Iodine. It is the
only place I could find it. We use it every day.
5 gms Iodine
1000ml 80% alcoh
Dissolve Iodine in 10 to 20 ml of the 80% alcoh. When dissolved add the
remainder of the alcohol. We run our slides down on the stainer to 95% alc
then they go into Alc-Iodine for 6-8 min. then wash and finish staining H&E.
The Iodine washes out and you are done. You do not need the hypo.
Lex Med Ctn
From: Gayle Callis [mailto:firstname.lastname@example.org]
Sent: Monday, July 12, 2004 2:31 PM
To: Cheryl Crowder; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Dezenkerizing slides
Hi Cheryl and all,
This answer has come up twice, but what is the percentage of iodine in the
70%alcohol IF one can't locate it in a book, etc?
>Ben - Years ago, when Zenker's was used by everyone, we used to put
>alcoholic iodine as the first station on the processor (after formalin).
>Then we added a sodium thiosulfate rinse to the stain routine. That took
>extra minute or 2. You might try that. Cheryl
>Cheryl Crowder, BA, HTL(ASCP)
>Department of Pathobiological Sciences
>School of Veterinary Medicine
>Louisiana State University
>Skip Bertman Drive
>Baton Rouge, LA 70803
>Histonet mailing list
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
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