strange sma staining


Dear All
For no apparent reason we are suddenly picking up nuclear staining on
SOME of our smooth muscle actins.  The only change has been the switch
from Basic Dab detection kits to I-View Dab for the Ventana (Nexes)
system that we have here.  We have increased the AB dilution
accordingly.  A bigger dilution to try and remove the nuclear staining,
in SOME cases also decreases the real staining. We use an appendix as a
control and this has not shown any non-specific staining during this
time. Has anybody had a similar experience or seen nuclear staining on
sma's?  If yes, please enlighten!  Our path's are baffled too.

Thanks in advance

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