I have found out from Vector (Novacastra) that their antibody is not compatible with B5 fixed tissues.  This is a problem for us since that is what our doctors like to use.  Right now we do the primary antibody at a 1:5 dilution after antigen retrieval in citric acid for 4 minutes in a pressure cooker, and do the detection on our Ventana NexEs.  Sometimes it looks great and at other times it is "puny".  Any advice concerning vendors, etc. would be greatly appreciated.  Thanks  in advance for everyone's help.

Martha Ward
Wake Forest University Baptist Medical Center