Re: bkg IHC
Which detection system are you using and are you using pretreatment?
>>> becky riesen 07/31/03 10:45AM >>>
I have specific staining in polys on my negative controls when the
section is a bone core from bone marrow biopsies. All other specimen
types seem to be fine. I have doubled the time in my H2O2 to 12
minutes. I have purchased new H2O2 in case I had an old bottle.
I'm increasing the time more, but I use an automated stainer and
this will lengthen the time of my entire run and I'm concerned about
getting too long here. I wonder if there are other suggestions,
solutions, procedures that you folks could share that may help my
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