we use the same Dako antibodies you do in monkey tissue (cynomolgus).
For both, we do AR with microwaving in citrate buffer, pH 6.
Dilution of primaries: kappa 1:2000 for 60 min
lambda 1:3000 for 30 min
Hope this helps.
Antje Marcantonio
Novartis Pharma AG
BU Transplantation Research
Basle/Switzerland
Bujakbarb@aol.com
28.07.2003 21:21
To: histonet@pathology.swmed.edu
cc:
Subject: Kappa, Lambda
Hi All,
Wonder if anyone can offer any suggestions for help with background staining of Kappa and Lambda on Monkey spleen and lymph node. The antibodies are from Dako: Kappa polyclonal/rabbit (A0191) and Lambda polyclonal/rabbit (A0193).