Re: Dilution buffers?
I use 50mM Tris-HCl, pH 7.4, with 1% BSA to dilute my antibodies; for washing, TBS with 0.03% Tween 20 works well. Hope this helps.
Nidal E Muvarak
Associate Research Specialist
Vascular Tissue Biomechanics Laboratory
Department of Biomedical Engineering
University of Wisconsin-Madison
1550 Engineering Dr.; Rm. 2158
Madison, WI 53706-1609
Lab: (608) 265-8921; Office: (608) 265-4205;
Home: (608) 256-7934; Cell: (608) 332-6068
----- Original Message -----
From: Sylvia Poulos
Date: Wednesday, July 30, 2003 1:13 pm
Subject: Re: Dilution buffers?
> we've had good luck diluting antibodies (primarily from Santa
> Cruz) in 1% BSA (sigma A4503) diluted in PBS. other BSA that
> we've tried hasn't been as good a diluent.
> Sylvia Poulos
> USDA-ARS-Animal Physiology Research Unit
> Athens, GA
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