I use 50mM Tris-HCl, pH 7.4, with 1% BSA to dilute my antibodies; for washing, TBS with 0.03% Tween 20 works well. Hope this helps.

Nidal E Muvarak
Associate Research Specialist
Vascular Tissue Biomechanics Laboratory
Department of Biomedical Engineering
University of Wisconsin-Madison
1550 Engineering Dr.; Rm. 2158
Madison, WI 53706-1609
Lab: (608) 265-8921; Office: (608) 265-4205; 
Home: (608) 256-7934; Cell: (608) 332-6068
http://vtb.bme.wisc.edu

----- Original Message -----
From: Sylvia Poulos 
Date: Wednesday, July 30, 2003 1:13 pm
Subject: Re: Dilution buffers?

> we've had good luck diluting antibodies (primarily from Santa 
> Cruz) in 1% BSA (sigma A4503) diluted in PBS.  other BSA that 
> we've tried hasn't been as good a diluent.
> 
> Sylvia Poulos
> USDA-ARS-Animal Physiology Research Unit
> Athens, GA 
> 
> 
>