Re: Deplasticize MMA

From:Gayle Callis

Yes, BUT they probably will have poor staining quality and may fall off the
slide.  Microtomed MMA sections are commonly deplasticized with 60C warmed
xylene several changes about 15 min each X 3 changes, but these are THIN,
3um to 5 um  sections.  Neil Hand (Theory and Practical Histological
Techniques, Gamble and Bancroft, 5th edition) does this on thin MMA
sections destined for immunostaining/other routine staining. Thicker ground
sections do not do well for IHC/routine staining. 

Anything thicker than 10 um or yours at 50um are better surfaced stained
while left on the glass slide.  Time for MMA removal may be greatly
lenthened by sections thickness and you may never get all the plastic out.

You did not say what you wanted to stain for?    


At 04:12 PM 7/30/2003 +0200, you wrote:
>Is it possible to deplasticize MMA thick sections (~50um) already glued on
>glass slides?
>If it is possible, would someone please relay me the procedure.
>Fabien Fuente
>Natural Implant
>Case Postale 908
>Parc Luminy Entreprise
>163 Av de Luminy
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)


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