Re: Alcian Blue
The dye is sold by Anatech and also by a Chinese
company. Both send their products to the Biological
Stain Commission for certification. If you look at
any recent issue of Biotechnic & Histochemistry you
will find sources in the "Stains recently certified"
section at the end.
There are also look-alike variants of alcian blue 8G,
notably the "pyridine variant." This is also OK.
See Churukian et al 2000. Biotech. Histochem.
75:147-150 and also the new "Conn's," Chapter 26.
With the pyridine variant the stain is not 100%
resistant to extraction by later acidic reagents,
as it is with alcian blue 8G, but for most purposes
this does not matter. See Conn's for explanations;
also A.Henwood 2002 in Biotech. Histochem. 77:93-94.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
"Richard Cartun (by way of Histonet)" wrote:
> I have been told that "Alcian Blue 8 GX" (used for histochemical
> staining at pH 1.0 & 2.5) is no longer available from Mallinckrodt. Is
> this true? Are there any satisfactory substitutes? Thank you.
> Richard Cartun
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