RE: nonspecific staining on neg. controls w/EDTA

From:"Favara, Cynthia (NIH/NIAID)"

My first question would be ...what quality control do you have for your EDTA

Do you have limits for temperature/time and is it monitored?
Is the EDTA pH checked? How often?

You probably have addressed these issues!

Just a place to start.

Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

-----Original Message-----
From: Tara Miller []
Sent: Tuesday, July 22, 2003 1:04 PM
Subject: nonspecific staining on neg. controls w/EDTA

Hi all,

I have been having difficulty trying to figure out why we occasionally have 
nonspecific staining on our negative controls when we use EDTA for HIER.  It

is not on any specific type tissue - we've had staining on skin, thyroid, 
ect.  We haven't changed any of our methods, but this has just cropped up as

a problem within the last month or so. Any suggestions?

Thanks in advance,
Tara Oakes HT
Central Dupage Hospital

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