My first question would be ...what quality control do you have for your EDTA
HIER?

Do you have limits for temperature/time and is it monitored?
Is the EDTA pH checked? How often?

You probably have addressed these issues!

Just a place to start.

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317


-----Original Message-----
From: Tara Miller [mailto:taratheht@hotmail.com]
Sent: Tuesday, July 22, 2003 1:04 PM
To: histonet@pathology.swmed.edu
Subject: nonspecific staining on neg. controls w/EDTA


Hi all,

I have been having difficulty trying to figure out why we occasionally have 
nonspecific staining on our negative controls when we use EDTA for HIER.  It

is not on any specific type tissue - we've had staining on skin, thyroid, 
ect.  We haven't changed any of our methods, but this has just cropped up as

a problem within the last month or so. Any suggestions?

Thanks in advance,
Tara Oakes HT
Central Dupage Hospital

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