RE: Which aqueous mounting media are you using and how do you cov erslip?

From:"Horn, Hazel V"

DAKO makes an aqueous mounting medium called Faramount.   It works great.
It's liquid at RT but it dries hard when coverslipped.
We love it.

Hazel Horn, HT/HTL (ASCP)
Histology Supervisor
Arkansas Children's Hospital

Phone - 501.364.4240
Fax - 501.364.3912 

-----Original Message-----
From: Gayle Callis [] 
Sent: Tuesday, July 29, 2003 11:23 AM
To: kevin williams;
Subject: Which aqueous mounting media are you using and how do you


How are you doing the coverslipping and which mounting media? 

Try a more generous drop os aqueous mounting media after wiping away excess
water around section.  I wonder if the water is degassing?  AquaMount
(Shandon Aqueous mounting media) is our favorite.  Some medias have a
thinner, watery consistency - these tend to give us more problems.  DAKO has
GlycerGel that is heated, but when applied to a dried section, was
excellent, plus it produces a hard set, no messy goo coming out around edges
or unstable coverslip. 

If we draw circles around section with a hydrophobic barrier pen, the flow
of media is impeded by hydrophobic goo - we get more bubbles or bubbles
form near/on goo.   We prefer to draw a line above and below section to
retain reagents during IHC. It is easier to blot reagents from tilted slide
with a squared off area/line than from circle, and when coverslipping, the
edges on either side of section are open, clear of goo - media flows evenly
from one side of slide to other. 



Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)


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