RE: Which aqueous mounting media are you using and how do you cov erslip?
DAKO makes an aqueous mounting medium called Faramount. It works great.
It's liquid at RT but it dries hard when coverslipped.
We love it.
Hazel Horn, HT/HTL (ASCP)
Arkansas Children's Hospital
Phone - 501.364.4240
Fax - 501.364.3912
From: Gayle Callis [mailto:firstname.lastname@example.org]
Sent: Tuesday, July 29, 2003 11:23 AM
To: kevin williams; email@example.com
Subject: Which aqueous mounting media are you using and how do you
How are you doing the coverslipping and which mounting media?
Try a more generous drop os aqueous mounting media after wiping away excess
water around section. I wonder if the water is degassing? AquaMount
(Shandon Aqueous mounting media) is our favorite. Some medias have a
thinner, watery consistency - these tend to give us more problems. DAKO has
GlycerGel that is heated, but when applied to a dried section, was
excellent, plus it produces a hard set, no messy goo coming out around edges
or unstable coverslip.
If we draw circles around section with a hydrophobic barrier pen, the flow
of media is impeded by hydrophobic goo - we get more bubbles or bubbles
form near/on goo. We prefer to draw a line above and below section to
retain reagents during IHC. It is easier to blot reagents from tilted slide
with a squared off area/line than from circle, and when coverslipping, the
edges on either side of section are open, clear of goo - media flows evenly
from one side of slide to other.
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
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