RE: Perfusing with zinc salt fixative

From:"Favara, Cynthia (NIH/NIAID)"

Did this a number of years ago, need to heparinize the mouse and

Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

flush out completely as the fix coagulates instead of crosslinking[ my recollection]. Morphology was not great in our opinion. May want to just perfuse with heparinized saline then harvest tissues and fix. Depends on what you want to accomplish.
I know this is rushed I am trying to get out for the weekend!
Good Luck!
-----Original Message-----
From: andrea portbury []
Sent: Wednesday, July 23, 2003 2:55 PM
To: histonet
Subject: Perfusing with zinc salt fixative

Hi there,
has anyone used the zinc salt fixative (NOT the zinc formalin fixative) to perfuse with? If so, could someone please tell me what buffer they used to clear the animal with prior to passing the fixative around? Usually I would use a phosphate buffer, however, this causes a precipitate to form in the fixative (which I am sure is not good for morphology).
Also, I have read where this fixative is best used on thin slices of tissue (rather than whole organs). Has anyone had any experience using this fixative on adult mouse brains? How long should I fix and post-fix for?
Thanks for any help you can give me.
Andrea Portbury, PhD.
Neuroscience Center,
University of Chapel Hill, NC

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