Barb,

Monoclonals are cleaner so try Dako's monoclonal Kappa & Lambda.  Don't feel
bad about these two antibodies being a bit dirty, they tend to be dirty for
everyone.  If your monoclonals are still dirty, try an Envision+ secondary
to help clean them up as well as whatever blocking you can throw in.

Good Luck,

Glen Dawson

-----Original Message-----
From: Bujakbarb@aol.com [mailto:Bujakbarb@aol.com]
Sent: Monday, July 28, 2003 2:22 PM
To: histonet@pathology.swmed.edu
Subject: Kappa, Lambda


Hi All,

Wonder if anyone can offer any suggestions for help with background staining
of Kappa and Lambda on Monkey spleen and lymph node. The antibodies are from
Dako: Kappa polyclonal/rabbit (A0191) and Lambda polyclonal/rabbit (A0193).

Thanks for your help,
Barb