Although the monoclonal kappa and lambda give a cleaner stain on human tissue, I'm not sure they will work on monkey spleen and lymph node.  Maybe going the ISH route may take care of the background... 

Sally Ann Drew-MT(ASCP)
Univ. of Wisconsin Hospital & Clinics
IHC/ISH Laboratory 
600 Highland Ave. VAH-DM223
Madison, WI 53792-2472
608-265-6596
sa.drew@hosp.wisc.edu


-----Original Message-----
From: Bujakbarb@aol.com [mailto:Bujakbarb@aol.com]
Sent: Monday, July 28, 2003 2:22 PM
To: histonet@pathology.swmed.edu
Subject: Kappa, Lambda


Hi All,

Wonder if anyone can offer any suggestions for help with background staining of Kappa and Lambda on Monkey spleen and lymph node. The antibodies are from Dako: Kappa polyclonal/rabbit (A0191) and Lambda polyclonal/rabbit (A0193).

Thanks for your help,
Barb