RE: Kappa, Lambda

From:"Sebree Linda A."

Try switching to monoclonal K and L.  Also be sure to block for endogenous biotin.

Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
FAX: (608)262-7174

-----Original Message-----
From: []
Sent: Monday, July 28, 2003 2:22 PM
Subject: Kappa, Lambda

Hi All,

Wonder if anyone can offer any suggestions for help with background staining of Kappa and Lambda on Monkey spleen and lymph node. The antibodies are from Dako: Kappa polyclonal/rabbit (A0191) and Lambda polyclonal/rabbit (A0193).

Thanks for your help,

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