RE: Kappa, Lambda
Try switching to monoclonal K and L. Also be sure to block for endogenous biotin.
Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
600 Highland Ave.
Madison, WI 53792
From: Bujakbarb@aol.com [mailto:Bujakbarb@aol.com]
Sent: Monday, July 28, 2003 2:22 PM
Subject: Kappa, Lambda
Wonder if anyone can offer any suggestions for help with background staining of Kappa and Lambda on Monkey spleen and lymph node. The antibodies are from Dako: Kappa polyclonal/rabbit (A0191) and Lambda polyclonal/rabbit (A0193).
Thanks for your help,
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