RE: H&E to Alcian Blue
I agree, de-stain in 1% Acid alcohol, then re-stain after washing in water
with AB
The nuclear stain shouldn't be too much of a problem.
Nick Kirk
Histopathology
Hinchingbrooke Hospital
Huntingdon
England
-----Original Message-----
From: John Kiernan [mailto:jkiernan@uwo.ca]
Sent: 23 July 2003 17:29
To: kevin williams
Cc: histonet@pathology.swmed.edu
Subject: Re: H&E to Alcian Blue
Yes. There's no need to remove the hemalum nuclear
stain, because alcian blue attaches to things
other than nuclei and is a different colour. If
you do remove the H and E you can do a red nuclear
counterstain after alcian blue.
--
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
kiernan@uwo.ca
http://publish.uwo.ca/~jkiernan/
_____________________________________________
kevin williams wrote:
>
> Dear All:
>
> Can anyone tell me if I can destain a H&E and restain
> it with an alcian blue.
>
> Yours faithfully,
>
> A. Kevin Williams.
>
>
> -----------------------------------------------------
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