RE: H&E to Alcian Blue

From:"Nick Kirk"

I agree, de-stain in 1% Acid alcohol, then re-stain after washing in water
with AB
The nuclear stain shouldn't be too much of a problem.

Nick Kirk
Hinchingbrooke Hospital

-----Original Message-----
From: John Kiernan []
Sent: 23 July 2003 17:29
To: kevin williams
Subject: Re: H&E to Alcian Blue

Yes. There's no need to remove the hemalum nuclear
stain, because alcian blue attaches to things
other than nuclei and is a different colour. If
you do remove the H and E you can do a red nuclear
counterstain after alcian blue.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
kevin williams wrote:
> Dear All:
> Can anyone tell me if I can destain a H&E and restain
> it with an alcian blue.
> Yours faithfully,
> A. Kevin Williams.
> -----------------------------------------------------
> Help STOP SPAM with the new MSN 8 and get 2 months

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