I've always found that keeping the slides that long in "Iron alum" leads to
much more non-specific "staining", especially in bone marrow trephines.
Here we incubate in "Iron alum" for 10 minutes maximum.
For some bone work we only incubate for 5 minutes due to the fact that the
bone has already been in acid for decalcification, which adds to the
background if you leave them in the Alum for 15 minutes.

Nick Kirk
Histopathology
Hinchingbrooke Hospital
Huntingdon
England.

-----Original Message-----
From: Cazares, Ruth [mailto:RCazares@schosp.org]
Sent: 22 July 2003 23:24
To: 'histonet@pathology.swmed.edu'
Subject: Gordon & Sweets Retic stain


Hello Again,

Does anyone out there have a variation of the Gordon & Sweets retic stain
that is quicker than the procedure in Freida Carsons book?  This procedure
calls for slides to remain in ferric ammonium sulfate for 15 minutes, is
anyone doing anything different?  All suggestions are appreciated.

Thanks in advance.

Ruth Cazares
Swedish Covenant Hospital
Chicago, IL



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