RE: GMA
Jenny,
Removal of the GMA is practically impossible. There are some published
methods for staining GMA sections eg:
A methyl blue/fuschin stain: Bennett, H.S., Wyrick, A.D., Lee, S.W., McNeil,
J.H., Stain Technology 51(2);71-97.
Castro,M.D.,(1985) "A Haematoxylin - Eosin Phloxine Stain for tissues
embedded in Glycol Methacrylate" J. Histotechn 8(1); 23-24.
Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318
http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html
-----Original Message-----
From: Jenny Molde [mailto:ctsmolde@capeheart.uct.ac.za]
Sent: Thursday, 24 July 2003 23:01
To: histonet@pathology.swmed.edu
Subject: GMA
Dear Histonetters
We are desperately trying to perfect the staining of routine
specials and H+E`s on Gma embedded sections. We are not being able
to get nicely stained sections. Is there anyway of removing Gma resin?
Any help will be appreciated. Many thanks.
Jenny Molde
University of Cape Town
Cardiovascular Research Unit
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