Need help embedding lung tisue

From:" Judy Strauss"

Dear Colleagues,

As a favor to a friend of a friend etc, I have been given inflated rat lung
fixed in 2.5% glutaraldehyde to embed in epon/araldite.  I have EM
experience but have never worked with lung.  The specimen will be used for
3D reconstruction so the pieces need to be larger than I routinely process. 
I need to know how to embed this research specimen and still keep the tissue
in its expanded state.  


Thanks for your help,
Judy



Judith Strauss
SUNY Upstate Medical University
Department of Orthopedic Surgery
IHP   room 3118
505 Irving Avenue
Syracuse, NY  13120

phone: (315) 464-9960
fax:  (315) 464-6638





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