Met AP2 Background staining


I'm trying to optimize this pab for use in IHC.  It's rabbit anti-MetAP-2 (methionine amiopeptidase-2) Zymed 71-7200.  I can't find much in the way of publications on using this for IHC - so I'm winging it.  I'm getting too much nonspecific staining.  I'm trying to use a human lymphoma and mouse spleen as negative controls, with mouse liver as a negative.  The only paper I could find (Kanno:Lab Invest, Volume 82(7) July 2002.893-901) suggested these tissues. I won't bore you with the gruesome details of my lack of success thus far, but if anyone out there has had any experience using this antibody or any other MetAP-2 antibody for IHC, please please help.  Thanks.

Jackie O'

Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotheraputics
Fax 847.938.3266

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