Immunostaining of murine aortic cross-sections

From:"sanjeev francis"

I am interested in immunostaining mouse aortic cross-sections to look for 
the tissue distribution of a particular protein.  I will be using a 
polyclonal Ab.  My question is how to prepare the tissue sections.  I have 
read that frozen sections preserve the antigenic integrity of tissues but 
have also heard that paraffin fixation is "milder" on the tissue.  Which 
technique should I use to optimize my immunostaining?

Sanjeev Francis, MD
Research Fellow
Weill Medical College of Cornell University

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