Immunostaining of murine aortic cross-sections
I am interested in immunostaining mouse aortic cross-sections to look for
the tissue distribution of a particular protein. I will be using a
polyclonal Ab. My question is how to prepare the tissue sections. I have
read that frozen sections preserve the antigenic integrity of tissues but
have also heard that paraffin fixation is "milder" on the tissue. Which
technique should I use to optimize my immunostaining?
Sanjeev Francis, MD
Weill Medical College of Cornell University
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