Hello all,
I am doing 4 Immuno-staining: Enkephalin, GFAP, Tyrosine Hydroxylase and GABA.
Doing the 4 at the same time, on the same brain, with the same protocol. I got stain in 3 of the 4.
Do some body have an idea why I don't get GABA staining at all? (we did diferents dilutions). Is something special with GABA?.
Thank you very much.
GABA is from Chemicon.

Luz Cardenas, MD
Post-doctoral Research Trainee
Department of Anatomy and Neurobiology
University of Tennessee Health Science Center
855 Monroe Avenue, Room 309/302
Memphis, TN 38163