Formol calcium versus NBF

From:"Chan Wai Kam"

Hi Histonetters,

Our lab has been using 10% formol calcium for fixation of rabbits bones
for years, as recommended by a visiting scientist.  I'm just wondering
whether there's any difference between using this compared to neutral
buffered formalin.  I've read that formol calcium is better for
preserving lipids but since our specimens are for normal processing in
paraffin which removes lipids, I'm wondering if it's really necessary to
keep to formol calcium.

Julee C 
Orthopaedic Surgery
National University of Singapore

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