Formalin fixation over the weekend?
We are currently in the process of collecting fetal tissue samples
for both IHC and DNA extraction. We have decided to formalin fix and
then paraffin embed the majority of our samples.
Our problem lies in the fact that we will not have access to tissue
samples until late on a Friday afternoon and will not be able to have
the tissues processed by the lab until Sunday evening/Monday morning
at the earliest. The tissues are all from termination of pregnancy so
are likely to be a little "fragile" and we are concerned about
storage in formalin over the weekend.
Would it be best to leave the tissue in 10% formalin for the weekend,
or should we fix for 24 hours and then transfer them to 70% ethanol?
It has also been suggested to us that we should fix and then
microwave the samples on a Friday night. If we do this, how should we
preserve the tissue over the weekend?
Any suggestions would be most appreciated.
University of Otago, New Zealand.
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