Employment Search for MSc Graduate
I hope that this use of the histonet mailserver is appropriate and please
accept my apologies for those that feel it is an inproper use of the
My name is Phillip Huff and I have recently completed my M.Sc. in
biochemistry with an emphasis on immunohistochemistry. I am searching for
a research assistant position working in any area dealing with
histochemistry, molecular biology or enzymology. In addition, if a
position exists, I am searching for a PhD studentship investigating any
aspect of adipogenesis, diabetes or the lipid aspects of arteriosclerosis.
The following resume will amplify on my successes as well as enhance other
significant areas of my background, most of which has a strong emphasis in
histochemistry and molecular biology research. Select highlights include:
#183# Experience with cryo-sectioning of whole tissues.
#183# Extensive experience immunohistochemical and immunocytochemical.
protocols for whole mount analysis in addition to Antigen
#183# Familiar with manual histological procedures.
Extensive molecular biology and enzymology experience.
#183# Responsible for overseeing current research project.
#183# Possess excellent communication and interpersonal skills.
#183# Willing to relocate in North America.
Additionally, I offer excellent analytical abilities and strong
organizational skills. My current work experience demands efficiency,
precision, and the ability to balance several different research projects
simultaneously. These qualities combined with my meticulous work ethic and
confidence, should enable me to make valuable contributions to any
What follows is my complete resume with contact information. Thank you for
taking the time to see if my qualifications meet your needs.
PHILLIP W. HUFF M.Sc.
# 726-21 Berkeley Place West Lethbridge, Alberta T1K 5N1 (403) 328-3899
To obtain a challenging position in the field of scientific research that
will utilize my education, work experience, and analytical abilities while
offering an opportunity for professional and personal growth.
▪Possess a Masters Degree in Biochemistry
▪5 years successful research in the field of lipid development and
▪Taught introductory chemistry labs and developed one half of a
fourth year biochemistry lab
▪Extensive experience in molecular biology techniques
▪Currently have 6 peer-reviewed publications and 7 contributions to
▪Experience in conducting presentations on research results to the
SKILLS AND ABILITIES
▪Molecular n Northern, and Southern blotting, cDNA cloning (RT-PCR),
PCR analysis, recombinant DNA procedures, RNA isolation/quantification,
bacterial cultures, bacterial transformations
▪Proteomics n fixed time enzyme assays, protein quantification,
protein purification via sucrose gradient centrifugation, SDS-PAGE,
Western blotting, cell suspension analysis, microsome preparation
▪Histochemistry n tissue collection, cryosectioning, fixation,
embedding, fluorescence immunocytochemistry and immunohistochemistry,
microscope image analysis
▪Additional Skills n infrared spectroscopy, thin layer
chromatography, lipid extractions, gas liquid chromatography, nuclear
magnetic resonance analysis
▪thoroughly researched literature dealing with adipogenesis for
▪developed database of all relevant articles (over 300) dealing with
▪data analysis using statistical software packages
▪Oral Communication n effectively presented M.Sc. results at two
international conferences, regularly deliver progress reports to
supervisors while conducting research projects, learned German language
quickly and efficiently to work with technicians during M.Sc. research,
taught three undergraduate courses while conducting M.Sc. research
▪Written Communication n successful acceptance of M.Sc. thesis,
currently writing three manuscripts based on M.Sc. research, partial
written contributions to five peer-reviewed articles
▪Hardware n able to work with PC and Macintosh computers, able to
install hard drives, RAM, CD-ROMs, and other hardware components
▪Software n software installation, troubleshooting, able to work
with Microsoft Office, Corel WordPerfect Suite, Sigma Plot, JMP IN, photo
analysis software, and Visual Basic, Abobe Acrobat
▪Fluent in French, working knowledge of German
▪Completed 16-hour St. John Ambulance Safety Oriented First Aid
course CPR n Level C
▪Certified for the safe handling of radioactive isotopes
Huff, P. W., Lozeman, F. J., Chris Kazala, E., Prozniak, C., Wegner, J.,
Deng, J. T., Laroche, A., Mir, P. S., Aalhus, J. and Weselake, R. J.
Stability of diacylglycerol acyltransferase in dehydrated bovine tissue.
Anal. Biochem. 318, 254-259.
Nykiforuk. C. L., Furukawa-Stoffer, T. L., Huff, P. W., Sarna, M.,
Laroche, A., Moloney, M. M. and Weselake, R. J. 2002. Characterization of
cDNAs encoding diacylglycerol acyltransferase from cultures of Brassica
napus and sucrose- mediated induction of enzyme biosynthesis. Biochim.
Biophys. Acta 1580, 95-109.
Wegner, J., Huff, P., Xie, C. P., Schneider, F., Teuscher, F., Mir, P. S.,
Mir, Z., Kazala, E. C., Weselake, R. J. and Ender, K. 2001. Relationship
of plasma leptin concentration to intramuscular fat content in beef from
crossbred Wagyu cattle. Can. J. Anim. Sci. 81, 451-457.
Wegner, J., Xie, C., Huff, P., Schneider, F., Mir, P.S., Mir, Z., Kazala,
E. C., Lozeman, F. J., Weselake, R. J. and Ender, K. 1999. Plasma leptin
levels and intramuscular fat content in beef from crossbreed Japanese
cattle. In: Proceedings of the 45th International Congress of Meat
Science, Vol. I, pp. 326-327, Japan Society for Meat Science and
Technology (August 1-6, Yokohama, Japan).
Nykiforuk, C. L., Huff, P. W., Taylor, D. C., Zou, J. T., Laroche, A. and
Weselake, R. J. 1998. Isolation and Sequence Analysis of a cDNA Encoding a
Ribosome-Associated p40 Protein from a Cell Suspension Culture of Brassica
napus L. cv Jet Neuf (Accession No. AF097522). Plant Physiology 118:1536
Nykiforuk, C. L., Huff, P. W., Furukawa-Stoffer, T. L., Taylor, D. C.,
Zou, J. T., Laroche, A. Weselake, R. J. 1998. Sucrose-mediated alterations
in lipid metabolism in microspore-derived cell suspension cultures of
oilseed rape. Advances in Plant Lipid Research. W. Sanchez, E.
Cerva-Olmedo, and E. Martinez-Forces editors. Pages 225-227.
JOURNAL SUBMISSIONS AND WORKS IN PROGRESS
Huff, P. W., Ren, M. Q., Lozeman, F., Weselake, R. J. and Wegner, J.
Expression of peroxisome proliferator-activated receptor (PPARg) mRNA in
adipose and muscle tissue of two different metabolic types of cattle.
Submitted to Canadian Journal of Animal Science.
Huff, P. W., Weselake, R. J. and Wegner, J. Development and metabolism and
intramuscular adipocytes in bovine skeletal muscle: a review. Current work
Huff, P.W., Wegner, J., Lozeman, F. J., Weselake, R. J.
Immunohistochemical localisation of preadipocyte factor-1: Potential
marker of preadipocytes in bovine muscle tissue. Current work in progress.
CONTRIBUTIONS TO CONFERENCE PROCEEDINGS
Huff, P. W., Wegner, J., Lozeman, F. and Weselake, R. J. 2002.
Immunohistochemical investigations of preadipocyte factor-1 expression in
genetically different cattle. 17th Annual Meeting of the Canadian Section
of the American Oil Chemistsi Society, September 28-30, Toronto, Canada.
Huff, P. W., Wegner, J., Ren, M. Q., Lozeman, F., Weselake, R. J. and
Ender, K. 2002. Expression of peroxisome proliferator-activated receptor
(PPARg) mRNA in adipose and muscle tissue of German Holstein and Charolais
cattle. ADSA-ASAS-CSAS Joint Annual Meeting, July 21-25, Quebec City,
Wegner, J., Albrecht, E., Huff, P., and Ender, K. 2000. Localization of
preadipocytes in bovine muscle tissue. 46th International Congress of Meat
Science and Technology, August 27 - September 1, Buenos Aries, Argentina.
Wiehler, W.B., Nykiforuk, C.L., Moloney, M.M., Laroche, A., Cianflone,
K.M., Kazala, E.C., Boehr, D., Huff, P.W. and Weselake, R.J. 1999. Effect
of mammalian proteins and triacylglycerol biosynthesis in Brassica napus
L. Agricultural Biotechnology Workshop, March 11-14, Banff, Alberta,
Nykiforuk, C. L., Huff, P., Furukawa-Stoffer, T., Taylor, D., Zou, J.,
Laroche, A., and Weselake, R. 1998. Investigation of sucrose-mediated
effects in a microspore-derived cell suspension culture of oilseed rape
exhibiting high oil formation capacity. The Annual Meeting of the Canadian
Section of the American Oil Chemistsi Society, October 4-5, Lethbridge,
Huff, P. W., Nykiforuk, C. L., Taylor, D. C., Zou, J., Laroche, A.,
Weselake, R. J. 1998. Isolation of a full-length cDNA from cell suspension
cultures of oilseed rape displaying enhanced triacylglycerol production.
1998. The Annual Meeting of the Canadian Section of the American Oil
Chemistsi Society, October 4-5, Lethbridge, Alberta, Canada.
C.L. Nykiforuk, J.M. Davoren, S.N. Little, P.W. Huff, A. Laroche, D.C.
Taylor and J-T. Zou. 1997. Investigation of gene expression in
microspore-derived cell suspension cultures of oilseed rape to identify
cDNAs encoding lipid biosynthetic enzymes. Supplement to Plant Physiol.
Master of Science in Biochemistry
The University of Lethbridge, Alberta
January 2001 - May 2003▪Research conducted in Dummerstorf, Germany
from August 2000 to July 2002
Bachelor of Science in Biochemistry
The University of Lethbridge, Alberta
September 1994 - April 1998
Laboratory of Dr. Randall J. Weselake The University of Lethbridge, Alberta
May -Dec 1998 / May- December 1999/ January 2000 and May 2003 n Present
Chemistry/Biochemistry Department The University of Lethbridge, Alberta
(Chemistry 1000, 2000, Biochemistry 4200) September 2002 n April 2003
Laboratory of Dr. Jochen Wegner FBN Dummerstorf, Germany
January - April 1999/July 2000 to December 2000
Career and Employment Services The University of Lethbridge, Alberta
May - August 1997
Best Student Presentation Canadian Section of the American Oil Chemistsi
Society 17th Meeting
September 28-30 2002 Toronto, Canada
Best Presentation Doctoral Student Day Research Institute for the Biology
of Farm Animals
June 2002 Dummerstorf, Germany
Dr. Randall J. Weselake
Professor of Biochemistry
Chemistry and Biochemistry Department, The University of Lethbridge, Alberta
Work: (403) 329-2303 Email: firstname.lastname@example.org
Dr. Jochen Wegner
Research Institute for the Biology of Farm Animals, Dummerstorf, Germany
Letter of reference is available upon request Email:
Dr. Fred J. Lozeman
Farmer, cow/calf producer (Claresholm, Alberta)
Adjunct Professor, Chemistry and Biochemistry Department, The University
of Lethbridge, Alberta
Work: (403) 625 1002 or (403) 625 4084 Email: email@example.com
<< Previous Message | Next Message >>