leg bones

From:Rohan Walker

Hi Histonet,

I have inherited some legs (rat) - snap frozen at time of collection and subsequently stored in a  -80 freezer.  I have been asked to measure bone lengths (tibia, fibia, femur). The catch is if there is one is that the entire leg has simply been removed from the pelvis, muscle and all and stored.  Now obviously I could simply thaw the leg and remove the muscle surgically but there are quite a few legs.  Might there be some alternative solution - I am thinking something along the lines of a solvent that I could stick all the legs into that would dissolve the muscle and not the bone?  Any suggestions?


Rohan Walker
Doctoral Student
Laboratory of Neuroimmunology
University of Newcastle
Callghan, N.S.W.  Australia.

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