Dear all histoneters,
I am doing IHC on mouse muscle recently. At the beginning of doing the work, I
embedded the tissue directly in the OCT on a mold at negative 20 dgeree. I had
bad morphology with space between muscle fibers and most of the muscle cell
have a big hole in the middle while inspected under microscope. After I read a
lot of histonet archive, I used LN2 to snap frozen muscle tissue. The
morphology of the tissue improved but there still got big holes in muscle
cells. I used Aceton as fixative after frozen section. I tried Zamboni#161#Os
fixative to fix sections for 20 minutes the other time, but unfortunately I
still got urgly results. I thought the reason was poor fixation of the
section. But I was afraid of using PFA would damage the antigenicity of the
tissue. Are there any other fixatives which can preserve the antigenicity well
enough I can use? It was a shame to say, I had spend almost one month on it.
It was bottle neck whick stop my work go ahead. Would you histoneters give me
some advice. If it is necessay,
Any idea will be appreciated.

Baowei Peng
Shanghai JiaoTong University,
P.R.C.


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