Renal biopsies

From:Lisa Black

Hello All,

I would like to have information from other sites regarding the way
renal biopsies are handled.  

At the lab where I work, the bx is received in Zeus fixative
(Michel's).  It is then placed in wash solution for 30 minutes.  At that
time a thin film of OCT is placed on a cork square and the bx is placed
on the cork.  The cork with the bx is then placed in a vial of -70#186# C
chilled isopentane and stored in the -70#186#C freezer until time to cut.

A thin film of OCT is placed onto a cryostat chuck.  Before it freezes
completely, the specimen is obtained from the freezer and the cork is
attached.  Sections are cut on the cryostat at 3 microns with 1 attached
to each slide.  15 slides are cut and allowed to dry for one hour prior
to staining.

This is the method of choice of the renal pathologist.  The theory is
to decrease freeze artifact.  Using this method causes great difficulty
in cutting a tiny specimen without any surronding OCT.  I have techs
that have worked at other sites that say there are much easier and more
efficient ways to perform this task.  Would those of you who routinely
cut renals be willing to give me advice?  Any information would be
greatly appreciated.

Thanks in advance.

Lisa Black
Carilion Consolidated Lab

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