Re: sectioning fresh prostates

From:Gayle Callis

Go to Thomas Scientific and look at the Thomas-Stadie-Riggs tissue slicer.  
The plastic slicer clamps the tissue for stable hold during slicing with
longer/sharp blade in a blade holder.  A simple device and you do not have
to prefix the tissue before slicing.    

We used these to slice brain and other large tissue samples.  If you have
their catalog, it is on page 1134 under microtome accessories. Website is 

At 07:31 PM 7/13/2003 -0500, you wrote:
>Question:We are interested in obtaining fresh tissue from radical
>prostatectomy specimens. For this we need to slice 2-3mm sections of the
>fresh prostate which is a rubbery organ measuring approximately 5x5x5cm
>(it can be somewhat smaller or larger). It is important to obtain whole
>slices that contain the entire dissection margin of the organ. The
>problem is that without fixation in formalin there is distortion of the
>tissue after sectioning so a really sharp knife and a way to hold the
>firmly is needed. Do you have a solution for this?
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)


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