Re: lipid controls

From:Geoff McAuliffe


    Your question did make it to the list, at least to my list. I was 
hoping someone with more lipid experience would answer but  ......

Jose Luis Palazon Fernandez wrote:

>Dear all
>Some days ago I posted this question, but have no answer. maybe It was not recieved by the list. I am doing lipid histochemistry and use delipidized control slides and run at same time with the sample slides. My question is, can we delipidize the control slides and then store them until the staining. 
    If the tissue is well fixed I don't see why not.

>If so, must them be frozen again?
    Why would they have to be frozen again?

> or have to be stored in a special way.
    Fixed tissue should be fine, I think. Unfixed tissue might decompose 
(?) and I think I would keep it  cold (frozen) with dessicant.

    This is an definitely an area where you should do the experiment for 
yourself with your tissues, lab proceedures, etc.  Then you will know 
for sure. This is part of the training for a Ph.D.


Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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