We had a similar problem when the techs were using freezing spray to chill
the small biopsies.


Jennifer MacDonald



----- Original Message -----
From: "Fred Underwood" 
To: 
Sent: Tuesday, July 08, 2003 9:36 AM
Subject: RE: hazing H&E's


> Hi Amy,
>     Don't overlook that the GI lab or office could be the problem.
Allowing the biopsy to dry before placing in fixative.  Leaving the specimen
jar on a window sill allowing it to overheat.  Maybe a jar of old or expired
fixative.  Many offices or
> employees of other departments are not as informed on how to handle
specimens as they should be.
>
> Fred
>
> -----Original Message-----
> From: 
> Sent: Monday, July 07, 2003 6:34 PM
> To: 
> Subject: hazing H&E's
>
>
>
>
>        Histonetters,
>
>        Hope everyone had a great holiday...... now back to work......
Here
> in the past couple of weeks my docs are telling me that they are seeing a
hazy
> bluish gray artifact over some of the tissue.  I had to see it to believe
it
> and sure enough there it was.  It almost looks like the slides are not
being
> deparaffinized enough so we worked on that and the problem is still here.
I
> don't feel that it is a staining problem because it would be only 2 3
slides out
> of a days worth of blocks (100+).  We bought a microwave and started
drying
> our slides in there, thought that that might be the problem but we did a
> comparison check on slides from the microwave and the drying oven.  Still
the problem
> is here..... The problem seems to be with our GI biopsies and so know I am
> wondering is there something going on with the tissue processor?  Any help
would
> be greatly appreciated......
>
>        Thanks,
>        Amy
>
>