Re: formalin fixation times
Our lab has a policy where all tissue is fixed for a
maximum of 24 hours in formalin. After that the
tissue is washed in running water for 10 minutes and
then placed in 70% ethanol until processed. Pellets
are fixed for the same amount of time. In our
processing we start at 70% ethanol, no fixation is
done on the processor at all. The rate of fixation I
read is ~1mm per hour at rt. Most of the problems I've
encountered with fixation is too much tissue and not
enough formalin, or tissue jammed into a cassette thus
causing uneven fixation. Between digestion or HIER, I
have very little trouble with the majority of the
antibodies I work with.
Institute for Cancer Prevention
Valhalla, New York
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