Re: chamberslides versus coverslips
I have also used Nunc's chambered coverglasses, they seem to work well for
inverted scope applications.
MT(ASCP) MS Microbiology
University of Montana
On 7/8/03 8:33, "Gayle Callis" wrote:
> You wrote:
> I'd appreciate your opinion on chamberslides vs. coated coversplis for
> neuronal cultures.
> David Moses
> Research fellow
> Monash Uni
> I have some questions
> What will be your way to examine these cultures, immunofluorescence,
> immunfluorescence for confocal scanning microscopy?
> Chamber slides are easier to handle in many ways than thin, fragile
> coverslips, but if you have to view the final stained and coverslipped
> GLASS chamber slide with confocal laser scanning microscope an inverted
> microscope then you can have the problem of the little chamber separations
> raising the coverslip away from the sample, changing the working distance
> of the objective. We use the NUNC chamber slides that have blue
> separations, rather than the removable gasket style.
> If it is standard fluorescence microscopy or light microscopy for
> immunostaining - whatever, then a chamber slide is very handy and certainly
> less work than necessity of coating coverslips, etc. Since you didn't say
> what kind of microscope you were using (inverted or upright), there are
> very handy glass bottom dishes for this purpose, the bottom of the dish
> (small petri style) is a coverslip of various diameters and thicknesses for
> culture, etc and fine work for confocal using inverted scopes, nonconfocal
> inverted microscopes, or using water immersion lenses on an upright
> microscope. So many ways to do things these days.
> Go to www.glassbottomdishes.com, they will send free samples, and some
> dishes are specially coated and sterile for culture work. MatTek is the
> name of the company.
> Gayle Callis
> Research Histopathology Supervisor
> Veterinary Molecular Biology - Marsh Lab
> Montana State University - Bozeman
> S. 19th and Lincoln St
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
> email: firstname.lastname@example.org
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