Re: PCNA on guinea pig
I've used Dako's PC10 anti PCNA on a wide varity of tissues ranging
from human to yeast . I dilute the primary at 1:100 and use their
Envision kit, the whole procedure takes about 45 minutes (or less). As
most of my tissues are fixed for no more than 72 hours I have not needed
to do antigen retrieval. I'd be happy to send you my SOP if you like.
Victoria Baker wrote:
>Is anyone working on PCNA with guinea pigs? I'm in a
>crunch and need to get an antibody that will react
>with guinea pig. Any info would be greatly
>Institute for Cancer Prevention
>Valhalla, New York 10595
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