Re: Ki-67 in mice

From:"Andi Kappeler" (by way of Histonet)

Dear Reinhard
For mice you might want to try clone TEC-3, a rat-a-mo Ki-67 mAb available
from DakoCytomation (M 7249). Works nicely on formalin-fixed cryostat
sections. Haven't tried it on FFPE yet, but according to the manufacturer it
should work (after HIER). Make sure to use a secondary that has been
absorbed with mo Ig (like Dako E 0468). For rats there is clone MIB-5
(mo-a-hu Ki-67) which should cross-react with rat Ki-67. We've played around
with it a couple of years ago, however we were never really happy. May be
there is a better alternative available now - and there is always BrdU. Good
Best regards

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

----- Original Message -----
Sent: Thursday, July 17, 2003 11:43 AM
Subject: Ki-67 in mice

> Hi Histonetters,
> please help me with the best monoclonal for Ki-67 in mice and rats for
FFPE tissue.
> Our Ki-67(MIB-1) for humans does not work. There must be a better solution
> PCNA (we don't want it !)
> Best regards
> Reinhard.
> PD Dr. med. Reinhard von Wasielewski

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