|From:||Susan Q Wells |
CD4 cat. # 554835 , CD 8 cat # 554854 Mouse anti rat (.1ug/ml ) from BD Pharmingen.
All reagents from DAKO except the primary.Run on Autostainer.
5 min. peroxidase block (with na azide)
30 min. primary incubation at room temp.
15 min in Link from LSAB2 HRP rat kit
15 minutes in label from LSAB2 HRP kit
DAB 5 min.
Does anyone have any experience and or protocol for demonstrating CD4 and CD 8 in fresh and or paraffin embedded tissue
in animal tissue.
Any information will be greatly appreciated.