RE: staining MMA sections

From:Chan Wai Kam

Hi Cathy,

I'm forwarding your e-mail to my colleague and let him get in touch with
you.  Thanks.

And thanks too to all who's written in with your helpful suggestions.


-----Original Message-----
From: Cathy Mayton [] 
Sent: Wednesday, July 09, 2003 9:56 PM
To: Histonet; Chan Wai Kam
Subject: staining MMA sections

Dear Julee,

It would be helpful if we could get more information regarding the
specimens.  After your second email, it sounds like there is a medical
device in the bone specimens.  More information on the animal model, the
implant site and metal device.  However, the processing of metal
implants in bone is well established in the literature and there are
many publications that are available that were published in the 90's.
Diane Sterchi and I published quite a few papers during that time that
may be beneficial to the work your lab is doing.  If you go to my
website I have listed many of my old publications.
Getting hold of these publications will lead you to others in all the
citations referenced in the papers.   If your colleague still has
questions he may want to post more direct questions on Histonet or I
would be happy to help him as well.  The processing of mineralized bone
can be very challenging and frustrating to the "newbie" but you have a
great support system already in place.  Barry Rittman, Gayle Callis and
others have already given some very helpful information.  The previous
publications will be very beneficial reading to your colleague.  The
nice thing is that there are folks around to point you in the right
direction.  When I started a new position in the early 80's leaving a
hospital histo lab for an orthopedic research lab, it was rude awakening
and at time there was no one to help, so it was all trial and error.
However, those trials and errors were put into publications so that
others did not have to struggle like our lab did in the early 80's.
Also, NSH has a subcommittee called the Hard Tissue Committee (HTC) that
may be beneficial to for your colleague to become a member of.  More
information on NSH and the HTC can be found at  

Hopefully this additional information will help. 

Take care,
"Quality Histology with a Personal Touch"

A GLP Compliant Laboratory

Cathy A. Mayton
Wasatch Histo Consultants, Inc.

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