RE: hazing H&E's

From:Pamela Marcum
When I lived in the Bay Area, Foster City CA, we found the tap water to at pH 8.3 to 8.7.  If I tried to blue sections it was a joke so we gave up and just used the water as the bluing agent.  Now I am outside Philadelphia and the water is closer to pH 7.0 -7.8 and I need to blue again.  so indeed checking the pH of the water in your area is important.  I found in my travels as a technical person that the pH of the water varies in all areas of the country. 
 
Also when the deionizer filters aren't changed or the distiller is off and not well cleaned the pH will change and again cause problems with staining, mixing of buffers and general usage.  It really mess up silver stains the worst and causes very significant shifts in the silver reactions.  Pam Marcum
-----Original Message-----
From: MARY T HODGES [mailto:hodges420@msn.com]
Sent: Monday, July 14, 2003 6:21 AM
To: Amy30histo@cs.com; histonet@pathology.swmed.edu; Marshall Terry Dr, Consultant Histopathologist
Subject: Re: hazing H&E's

Dr. Terry
I stand by the theory of PH proven by Ventana Medical here in Tucson. PH of water is more significant then most people believe.
Tere Hodges
NorthWest Medical Center
Tucson, Arizona
----- Original Message -----
From: Marshall Terry Dr, Consultant Histopathologist
To: Amy30histo@cs.com ; histonet@pathology.swmed.edu
Sent: Monday, July 14, 2003 6:30 AM
Subject: RE: hazing H&E's

Several people, as has happened before, referred to cold as a (the) cause of this phenomenon. As before, nobody has suggested a mechanism by which cold water induces this odd appearance.
In our lab., cold weather is held with lingering suspicion as a cause, and certainly, particularly in winter, which is most of the year in UK, the water is f-f-f-freezing.
But if this is the answer, what is going on?
 

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire
 England
        terry.marshall@rothgen.nhs.uk

-----Original Message-----
From: Amy30histo@cs.com [mailto:Amy30histo@cs.com]
Sent: 14 July 2003 03:16
To: histonet@pathology.swmed.edu
Subject: hazing H&E's



      Thanks to all who responded to my hazing H&E's problem..... I've got a lot of research/work to do to solve this problem.   We had this one time before about 2 years ago and the problem went away before we could really figure it out.  Again thanks for all the help from everyone.


      Amy
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