RE: hazing H&E's

From:Claye Clyatt

Same here.  We would go for months without seeing this problem.  We suddenly realized that it reoccurred each winter as the temperature fell.  We adjusted and monitored the water temperature to a constant setting and the problem never came back.  


Claye Clyatt
Chief Histotechnologist
Department of Pathology 
Room #BF119
Medical College of Georgia
Augusta, Ga 30912
office   (706) 721-3630
pager  (706) 721-7243-1132

>>> "Soto, Roxanne"  07/09/03 01:39PM >>>
We had this problem and it turned out to be the temperature of the water
washes on the was too cold.
Roxanne Soto HT (ASCP)
AP Supervisor

-----Original Message-----
From: Ellen Clausen-Simon [mailto:ECSIMON@NORTHERNHEALTH.ORG] 
Sent: Wednesday, July 09, 2003 12:23 PM
Subject: Re: hazing H&E's

Sounds familiar. We had the same problem, and changed out the entire
processor, replaced with all new reagents. No recycled stuff. Hope this

>>>  07/07/03 06:33PM >>>

       Hope everyone had a great holiday...... now back to work......  Here 
in the past couple of weeks my docs are telling me that they are seeing a
bluish-gray artifact over some of the tissue.  I had to see it to believe it

and sure enough there it was.  It almost looks like the slides are not being

deparaffinized enough so we worked on that and the problem is still here.  I

don't feel that it is a staining problem because it would be only 2-3 slides
of a days worth of blocks (100+).  We bought a microwave and started drying 
our slides in there, thought that that might be the problem but we did a 
comparison check on slides from the microwave and the drying oven.  Still
the problem 
is here..... The problem seems to be with our GI biopsies and so know I am 
wondering is there something going on with the tissue processor?  Any help
be greatly appreciated......


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