RE: frozen section on mouse muscle

From:mikesafron

If you coat the tissue with talc and place tissue on top of a
partially frozen OCT compound bead prior to complete freezing of
tissue in Liquid N2 the freeze artifact will be greatly reduced.

Michael A. Safron A.S., HT(ASCP)
Manager, Histology
WIL Research Laboratories Inc.


---- Original Message ----
From: pengbaowei@21cn.com
To: histonet@pathology.swmed.edu
Subject: RE: frozen section on mouse muscle
Date: Wed, 16 Jul 2003 09:05:16 +0800 (CST)

>Dear all histoneters,
>I am doing IHC on mouse muscle recently. At the beginning of doing
>the work, I
>embedded the tissue directly in the OCT on a mold at negative 20
>dgeree. I had
>bad morphology with space between muscle fibers and most of the
>muscle cell
>have a big hole in the middle while inspected under microscope. After
>I read a
>lot of histonet archive, I used LN2 to snap frozen muscle tissue. The
>morphology of the tissue improved but there still got big holes in
>muscle
>cells. I used Aceton as fixative after frozen section. I tried
>Zamboni#161#Os
>fixative to fix sections for 20 minutes the other time, but
>unfortunately I
>still got urgly results. I thought the reason was poor fixation of
>the
>section. But I was afraid of using PFA would damage the antigenicity
>of the
>tissue. Are there any other fixatives which can preserve the
>antigenicity well
>enough I can use? It was a shame to say, I had spend almost one month
>on it.
>It was bottle neck whick stop my work go ahead. Would you histoneters
>give me
>some advice. If it is necessay,
>Any idea will be appreciated.
>
>Baowei Peng
>Shanghai JiaoTong University,
>P.R.C.
>
>
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