We always thawed in cold PBS with no damage.  We found it good practice to
have a bottle of PBS always handy in the refrigerator.  Hope this helps.

Juan C. Gutierrez, HT(ASCP)
Histology Supervisor
Christus Santa Rosa Hospital
(210)704-2533
Juan_Gutierrez@srhc.iwhs.org


-----Original Message-----
From: Bruce Abaloz [mailto:brucea@unimelb.edu.au]
Sent: Thursday, July 17, 2003 12:27 AM
To: histonet@pathology.swmed.edu
Subject: Tissue in OCT - how to "Thaw??? HELP!!


Hello Histonetters/Colleagues, 

I have a piece of 'Pouch Young' (Wallaby) tissue approx. 1mm. in size that
was fixed in 4%  PFA then frozen in OCT. I now want to do 'WHOLE MOUNT
IMMUNO. & am wondering what procedure IS best to 'thaw' the tissue with
MINIMAL damage to the cells - OR anyone with previous experience RE: this
situation can offer advice. Thankyou in advance,


Gratiana ( g.wijayanti@pgrad.unimelb.edu.au )
-- 
BRUCE ABALOZ                           PH:61383446282
HISTOLOGIST                            FAX:61383447909 
DEPT.of ZOOLOGY                        EMAIL: brucea@unimelb.edu.au
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