RE: Processor failure
A word of caution. I have tried running tissue thru the purge cycle on the VIP. With the retort chamber being heated, the xylene gets quite hot. The tissue went from being sponge like to brick like.
From: Nick Kirk
Sent: Wednesday, July 16, 2003 6:38 PM
To: Joey Wilkie
Subject: RE: Processor failure
Processor failureOh dear, sounds like a bit of a disaster.
My advice would to "reverse process".
The most important thing you need to do is remove any wax that is present in
the tissue. There is bound to be a small amount of impregnation even after
what the tissue has been through.
This is essential before any repeat processing is attempted.
I would put the blocks through the "flush cycle" on the VIP, i.e. xylenes
(or substitute) followed by alcohols, preferably at 45 degrees centigrade,
to ensure complete wax removal and impregnation by the alcohol.
This should get your tissue back to a state where it can then be re hydrated
safely with minimal tissue damage.
Fixation would not appear to be a problem so you could miss that step out
when you re process to speed things up a bit.
Head Biomedical Scientist
From: Joey Wilkie [ mailto:JWilkie@stmarygj.com]
Sent: 16 July 2003 17:38
Subject: Processor failure
Last week our embedded came in to embed and found that the tissue was not
fixed. After some investigation, we found that the some of the carbouys on
the processors had been misplaced. Our tissue had gone through a 10%
formalin, a 100% alcohol , 50% alcohol , 80% alcohol , two 95% alcohols,
100% alcohol, back into a 10% formalin and then into two xylenes, and then
into four paraffin baths. We are using a VIP processor.
Now the question is how do we go about reprocessing the tissue. Any help
that you can give us would be greatly appreciated.
St. Mary's Hospital
Grand Junction, Colorado
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