RE: Processor failure

From:Fred Underwood

A word of caution.  I have tried running tissue thru the purge cycle on the VIP.  With the retort chamber being heated, the xylene gets quite hot.  The tissue went from being sponge like to brick like.

	-----Original Message-----
From: Nick Kirk  
Sent: Wednesday, July 16, 2003 6:38 PM
To: Joey Wilkie 
Subject: RE: Processor failure

	Processor failureOh dear, sounds like a bit of a disaster. 
My advice would to "reverse process". 
The most important thing you need to do is remove any wax that is present in 
the tissue. There is bound to be a small amount of impregnation even after 
what the tissue has been through. 
This is essential before any repeat processing is attempted. 
I would put the blocks through the "flush cycle" on the VIP, i.e. xylenes 
(or substitute) followed by alcohols, preferably at 45 degrees centigrade, 
to ensure complete wax removal and impregnation by the alcohol. 
This should get your tissue back to a state where it can then be re hydrated 
safely with minimal tissue damage. 
Fixation would not appear to be a problem so you could miss that step out 
when you re process to speed things up a bit. 

	Good luck 

	Nick Kirk 
Head Biomedical Scientist 
Hinchingbrooke Hospital 
       Original Message      
  From: Joey Wilkie [] 
  Sent: 16 July 2003 17:38 
  To: '' 
  Subject: Processor failure 

	  Last week our embedded came in to embed and found that the tissue was not 
fixed.  After some investigation, we found that the some of the carbouys on 
the processors had been misplaced.  Our tissue had gone through a 10% 
formalin, a 100% alcohol , 50% alcohol , 80% alcohol , two 95% alcohols, 
100% alcohol, back into a 10% formalin and then into two xylenes, and then 
into four paraffin baths.  We are using a VIP processor. 

	  Now the question is how do we go about reprocessing the tissue.  Any help 
that you can give us would be greatly appreciated. 

	  Thanks, Joey 
  St. Mary's Hospital 
  Grand Junction, Colorado 
  This electronic message and all contents contain information from St. 
Mary's Hospital  which may be attorney client privileged, confidential or 
otherwise protected from disclosure.  The information is intended to be for 
the addressee only.  If you are not the addressee, any disclosure, copy, 
distribution or use of the contents of this message is prohibited.  If you 
have received this electronic message in error, please notify the sender 
immediately and destroy the original message and all copies.  Thank you." 

<< Previous Message | Next Message >>