RE: Processor failure

From:

Tony Henwood

Processor failure

Wow! What a muck up!

I would recommend the procedure given in the last issue of Histologic (May 2003) by Michael Johnson. We have tried it and it works very well.

In summary:

Melt the wax blocks

Gently press out excess wax from the larger blocks, blot the smaller biopsies and the larger ones to remove excess wax.

Immediately place in 10% formalin and process as usual.

Regards

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html

-----Original Message-----
From: Joey Wilkie [mailto:JWilkie@stmarygj.com]
Sent: Thursday, 17 July 2003 2:38
To: 'histonet@pathology.swmed.edu'
Subject: Processor failure

Last week our embedded came in to embed and found that the tissue was not fixed. After some investigation, we found that the some of the carbouys on the processors had been misplaced. Our tissue had gone through a 10% formalin, a 100% alcohol , 50% alcohol , 80% alcohol , two 95% alcohols, 100% alcohol, back into a 10% formalin and then into two xylenes, and then into four paraffin baths. We are using a VIP processor.

Now the question is how do we go about reprocessing the tissue. Any help that you can give us would be greatly appreciated.

Thanks, Joey
St. Mary's Hospital
Grand Junction, Colorado
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