RE: Processor failure

From:Tony Henwood

Processor failure
Wow! What a muck up!
I would recommend the procedure given in the last issue of Histologic (May 2003) by Michael Johnson. We have tried it and it works very well.
In summary:
Melt the wax blocks
Gently press out excess wax from the larger blocks, blot the smaller biopsies and the larger ones to remove excess wax.
Immediately place in 10% formalin and process as usual.

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: Joey Wilkie []
Sent: Thursday, 17 July 2003 2:38
To: ''
Subject: Processor failure

Last week our embedded came in to embed and found that the tissue was not fixed.  After some investigation, we found that the some of the carbouys on the processors had been misplaced.  Our tissue had gone through a 10% formalin, a 100% alcohol , 50% alcohol , 80% alcohol , two 95% alcohols, 100% alcohol, back into a 10% formalin and then into two xylenes, and then into four paraffin baths.  We are using a VIP processor.

Now the question is how do we go about reprocessing the tissue.  Any help that you can give us would be greatly appreciated.

Thanks, Joey
St. Mary's Hospital
Grand Junction, Colorado
This electronic message and all contents contain information from St. Mary's Hospital  which may be attorney-client privileged, confidential or otherwise protected from disclosure.  The information is intended to be for the addressee only.  If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited.  If you have received this electronic message in error, please notify the sender immediately and destroy the original message and all copies.  Thank you."

This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please
delete it and notify the sender.

Views expressed in this message and any attachments are those
of the individual sender, and are not necessarily the views of The
Children's Hospital at Westmead

This footnote also confirms that this email message has been
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any
consequential damage resulting from email containing computer

<< Previous Message | Next Message >>