RE: Processor failure

From:"Favara, Cynthia (NIH/NIAID)"

Processor failure
What a nightmare!!
I would reverse process ie. paraffin x 4, xylene x 2, 100% ETOH x 2, 95%ETOH x 2. Then put it though starting with the 95% ETOH to paraffin.
The time in the ETOH already is likely to make tissue a bit brittle but should be able to get readable sections.
Good Luck!
Cynthia Favara
-----Original Message-----
From: Joey Wilkie []
Sent: Wednesday, July 16, 2003 10:38 AM
To: ''
Subject: Processor failure

Last week our embedded came in to embed and found that the tissue was not fixed.  After some investigation, we found that the some of the carbouys on the processors had been misplaced.  Our tissue had gone through a 10% formalin, a 100% alcohol , 50% alcohol , 80% alcohol , two 95% alcohols, 100% alcohol, back into a 10% formalin and then into two xylenes, and then into four paraffin baths.  We are using a VIP processor.

Now the question is how do we go about reprocessing the tissue.  Any help that you can give us would be greatly appreciated.

Thanks, Joey
St. Mary's Hospital
Grand Junction, Colorado
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