RE: Humidity and Xylene Subs
I wouldn't be surprised if the bags can be heated to drive off the
water and be reused. We do this routinely with both the molecular
sieves and silica gel beads we use for desiccating things. Works
great. 200 deg C or so for 2 to 4 hours is fine. Or, if your
desiccant has indicator in it (blue = good), whenever the indicator
is blue again.
Phil
Dear Gary:
This is the unchecked info I was given by the Tec Support at Richard
Allen, I have called the actual makers of the beads and I am wating
to hear back from them, I will give you more complete information
once I have it.
Yours faithfully,
A.Kevin Williams.
>From: Gary Gill
>To: 'Pamela Marcum' , kevin williams , maria.geraci@spcorp.com,
>histonet@pathology.swmed.edu
>Subject: RE: Humidity and Xylene Subs
>Date: Wed, 09 Jul 2003 14:53:09 -0500
>
>Check with the vendor. To my knowledge, such molecular sieves cannot be
>regenerated simply by placing them in an oven. A technical support rep of
>UOP, a major manufacturer of these beads, described the recommended process.
>It requires equipment that laboratories don't ordinarily have. Vendors
>can't stay in business by selling single units of items that can be used
>forever. There's no profit in loss.
>
>Gary Gill
>
>-----Original Message-----
>From: Pamela Marcum [mailto:pmarcum@polysciences.com]
>Sent: Wednesday, July 09, 2003 2:14 PM
>To: kevin williams; maria.geraci@spcorp.com; histonet@pathology.swmed.edu
>Subject: RE: Humidity and Xylene Subs
>
>
>Kevin,
>
>Drying the bags sounds like a good idea and would save money. However, the
>oven had better be under a hood or the fumes will drive you out and are
>dangerous.
>
>Pam Marcum
>
>-----Original Message-----
>From: kevin williams [mailto:akwilliams75@hotmail.com]
>Sent: Wednesday, July 09, 2003 2:35 PM
>To: maria.geraci@spcorp.com; histonet@pathology.swmed.edu
>Subject: RE: Humidity and Xylene Subs
>
>
>
>Dear Maria:
>
>I have just found some info on a company called Sun Cycle Technologies.
>
>1866-786-2925
>
>They appartently have some absorbant bags that you can put in the oven after
>use, dry out and re-use.
>
>Sounds intresting I will be looking into both options.
>
>Thanks for your help,
>
>A.Kevin Williams
>
>
>
> >From: "Geraci, Maria"
> >To: "'kevin williams'"
> >Subject: RE: Humidity and Xylene Subs
> >Date: Wed, 9 Jul 2003 11:32:57 -0400
> >
> >Hi Kevin,
> >
> >The company I order from is called: ACROS Organics. I believe the order
> >number is 197245000. They are called: Molecular Sieves 3A, 4 to 8 mesh.
> >
> >Maria
> >
> >Dear Maria:
> >
> >Thanks for getting back to me I am having a lot of problems with the water.
>
> >I though my reagent bottles may be contaminated, but this sounds tike the
> >humidity. I was wondering if you could tell me the company name so I can do
>
> >some investigating.
> >
> >Thanks for your help.
> >
> >Yours faithfully,
> >
> >A.Kevin Williams.
> >
> >
> >
> > >From: "Geraci, Maria"
> > >To: "'kevin williams'"
> > >Subject: RE: Humidity and Xylene Subs
> > >Date: Mon, 7 Jul 2003 13:56:22 -0400
> > >
> > >Hi Kevin,
> > >
> > >I don't know if it will work on xylene substitutes, I've never tried. We
> > >use recycled xylene in our lab and it works on the recycled xylene. The
> >mesh
> > >size is related to the chemical you are trying to dry out. I had to talk
> > >with the manufacturer in order to know what size sieve to use. The sieves
>
> > >are actual ceramic type beads that are placed at the bottom of your
>xylene
> > >container and they absorb water to keep the xylene dry. They do not
> > >actually come in contact with your slides. It is hard to know when they
>are
> >
> > >exhausted so when you start getting water droplets on your slides, you
>know
> >
> > >it's time to change the beads. Hopefully you'll do a test run each time
>you
> >
> > >process so you don't ruin your actual slides. These sieves can be used in
>
> > >100% alcohol also to prevent water absorption. They work really well.
> > >
> > >Maria
> > >
> > >-----Original Message-----
> > >From: kevin williams [mailto:akwilliams75@hotmail.com]
> > >Sent: Monday, July 07, 2003 1:27 PM
> > >To: maria.geraci@spcorp.com
> > >Subject: RE: Humidity and Xylene Subs
> > >
> > >
> > >This message was scanned by MIMEsweeper and determined to possibly be
>Spam
> > >mail. This scanning was performed to assist you in dealing more
>effectively
> >
> > >with this type of message in the future. You may read the message and
> > >determine what course of action is appropriate to take. The below link
>will
> >
> > >take you to an internal Webpage with several options to automatically
> > >process messages that are determined to be Spam. Please review these
> >options
> > >and decide which solution is appropriate in your situation:
> > >http://corporate/cit/Rescenter/email/spamrule.pdf
> > >http://corporate/cit/Rescenter/email/spamrule.pdf
> > >
> > >
> > >
> > >
> > >
> > >
> > >Dear Maria:
> > >
> > >I am intrested to know if you can use this for Xylene sub, and it apears
>as
> >
> > >though it is a mesh that you place on the surface, is this correct? Do
>you
> > >take it off when the slides are being processed? Or do I have totally the
>
> > >wrong idea.
> > >
> > >Thanks for your help.
> > >
> > >Yours faithfully,
> > >
> > >A.Kevin Williams.
> > >
> > >
> > >
> > > >From: "Geraci, Maria"
> > > >To: 'Linda Stirpe' , HistoNet Server
> > > >Subject: RE: Humidity and Xylene Subs
> > > >Date: Mon, 07 Jul 2003 09:21:05 -0400
> > > >
> > > >Hi Linda,
> > > >
> > > >We have the same problem in our laboratory in the summer and I use
> > >Molecular
> > > >Sieves 3A, 4-8 mesh put out by ACROS Organics. I believe the order
>number
> >
> > > >is 197245000. And yes, they really do work. I just add them to the
>xylene
> >
> > > >and I change them when I change the xylene.
> > > >
> > > >Good Luck
> > > >
> > > >Maria
> > > >
> > > >Maria A. Geraci
> > > >Pathology/Immunohistochemistry
> > > >Schering-Plough Research Institute
> > > >P.O. Box 32 Route 94 South
> > > >Lafayette, New Jersey 07848-0032
> > > >
> > > >Phone: (973) 940-4284
> > > >FAX: (973) 940-4183
> > > >maria.geraci@spcorp.com
> > > >
> > > >
> > > >
> > > >-----Original Message-----
> > > >From: Linda Stirpe [mailto:lpstirpe@yahoo.com]
> > > >Sent: Wednesday, July 02, 2003 7:51 PM
> > > >To: HistoNet Server
> > > >Subject: Humidity and Xylene Subs
> > > >
> > > >
> > > >Hi Fellow Histonetters,
> > > >
> > > >Now that summer and humidity is upon us our lab is
> > > >having the usual "bleeding eosin" problem. Last year
> > > >we were staining by hand but this year we were lucky
> > > >enough to get an automatic stainer which we thought
> > > >would take care of the problem - guess again. We are
> > > >using Richard Allan Clear Rite 3 which I know loves to
> > > >absorb water. I remember some literature about
> > > >"absorbant beads" to place in the Xylene Subs to help
> > > >with this problem. Since I cannot find the literature
> > > >- does anyone know the name, supplier, price, etc. and
> > > >most important do they work? Thanks!
> > > >
> > > >Have a great holiday!
> > > >
> > > >Linda Stirpe
> > > >Corning Hospital
> > > >Corning, NY 14830
> > > >
> > > >__________________________________
> > > >Do you Yahoo!?
> > > >SBC Yahoo! DSL - Now only $29.95 per month!
> > > >http://sbc.yahoo.com
> > > >
> > > >
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Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
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