RE: Humidity and Xylene Subs

From:kevin williams

Dear Gary:

This is the unchecked info I was given by the Tec Support at Richard Allen, I have called the actual makers of the beads and I am wating to hear back from them, I will give you more complete information once I have it.

Yours faithfully,

A.Kevin Williams.

>From: Gary Gill
>To: 'Pamela Marcum' , kevin williams , maria.geraci@spcorp.com, histonet@pathology.swmed.edu
>Subject: RE: Humidity and Xylene Subs
>Date: Wed, 09 Jul 2003 14:53:09 -0500
>
>Check with the vendor. To my knowledge, such molecular sieves cannot be
>regenerated simply by placing them in an oven. A technical support rep of
>UOP, a major manufacturer of these beads, described the recommended process.
>It requires equipment that laboratories don't ordinarily have. Vendors
>can't stay in business by selling single units of items that can be used
>forever. There's no profit in loss.
>
>Gary Gill
>
>-----Original Message-----
>From: Pamela Marcum [mailto:pmarcum@polysciences.com]
>Sent: Wednesday, July 09, 2003 2:14 PM
>To: kevin williams; maria.geraci@spcorp.com; histonet@pathology.swmed.edu
>Subject: RE: Humidity and Xylene Subs
>
>
>Kevin,
>
>Drying the bags sounds like a good idea and would save money. However, the
>oven had better be under a hood or the fumes will drive you out and are
>dangerous.
>
>Pam Marcum
>
>-----Original Message-----
>From: kevin williams [mailto:akwilliams75@hotmail.com]
>Sent: Wednesday, July 09, 2003 2:35 PM
>To: maria.geraci@spcorp.com; histonet@pathology.swmed.edu
>Subject: RE: Humidity and Xylene Subs
>
>
>
>Dear Maria:
>
>I have just found some info on a company called Sun Cycle Technologies.
>
>1866-786-2925
>
>They appartently have some absorbant bags that you can put in the oven after
>use, dry out and re-use.
>
>Sounds intresting I will be looking into both options.
>
>Thanks for your help,
>
>A.Kevin Williams
>
>
>
> >From: "Geraci, Maria"
> >To: "'kevin williams'"
> >Subject: RE: Humidity and Xylene Subs
> >Date: Wed, 9 Jul 2003 11:32:57 -0400
> >
> >Hi Kevin,
> >
> >The company I order from is called: ACROS Organics. I believe the order
> >number is 197245000. They are called: Molecular Sieves 3A, 4 to 8 mesh.
> >
> >Maria
> >
> >Dear Maria:
> >
> >Thanks for getting back to me I am having a lot of problems with the water.
>
> >I though my reagent bottles may be contaminated, but this sounds tike the
> >humidity. I was wondering if you could tell me the company name so I can do
>
> >some investigating.
> >
> >Thanks for your help.
> >
> >Yours faithfully,
> >
> >A.Kevin Williams.
> >
> >
> >
> > >From: "Geraci, Maria"
> > >To: "'kevin williams'"
> > >Subject: RE: Humidity and Xylene Subs
> > >Date: Mon, 7 Jul 2003 13:56:22 -0400
> > >
> > >Hi Kevin,
> > >
> > >I don't know if it will work on xylene substitutes, I've never tried. We
> > >use recycled xylene in our lab and it works on the recycled xylene. The
> >mesh
> > >size is related to the chemical you are trying to dry out. I had to talk
> > >with the manufacturer in order to know what size sieve to use. The sieves
>
> > >are actual ceramic type beads that are placed at the bottom of your
>xylene
> > >container and they absorb water to keep the xylene dry. They do not
> > >actually come in contact with your slides. It is hard to know when they
>are
> >
> > >exhausted so when you start getting water droplets on your slides, you
>know
> >
> > >it's time to change the beads. Hopefully you'll do a test run each time
>you
> >
> > >process so you don't ruin your actual slides. These sieves can be used in
>
> > >100% alcohol also to prevent water absorption. They work really well.
> > >
> > >Maria
> > >
> > >-----Original Message-----
> > >From: kevin williams [mailto:akwilliams75@hotmail.com]
> > >Sent: Monday, July 07, 2003 1:27 PM
> > >To: maria.geraci@spcorp.com
> > >Subject: RE: Humidity and Xylene Subs
> > >
> > >
> > >This message was scanned by MIMEsweeper and determined to possibly be
>Spam
> > >mail. This scanning was performed to assist you in dealing more
>effectively
> >
> > >with this type of message in the future. You may read the message and
> > >determine what course of action is appropriate to take. The below link
>will
> >
> > >take you to an internal Webpage with several options to automatically
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> > >http://corporate/cit/Rescenter/email/spamrule.pdf
> > >
> > >
> > >
> > >
> > >
> > >
> > >Dear Maria:
> > >
> > >I am intrested to know if you can use this for Xylene sub, and it apears
>as
> >
> > >though it is a mesh that you place on the surface, is this correct? Do
>you
> > >take it off when the slides are being processed? Or do I have totally the
>
> > >wrong idea.
> > >
> > >Thanks for your help.
> > >
> > >Yours faithfully,
> > >
> > >A.Kevin Williams.
> > >
> > >
> > >
> > > >From: "Geraci, Maria"
> > > >To: 'Linda Stirpe' , HistoNet Server
> > > >Subject: RE: Humidity and Xylene Subs
> > > >Date: Mon, 07 Jul 2003 09:21:05 -0400
> > > >
> > > >Hi Linda,
> > > >
> > > >We have the same problem in our laboratory in the summer and I use
> > >Molecular
> > > >Sieves 3A, 4-8 mesh put out by ACROS Organics. I believe the order
>number
> >
> > > >is 197245000. And yes, they really do work. I just add them to the
>xylene
> >
> > > >and I change them when I change the xylene.
> > > >
> > > >Good Luck
> > > >
> > > >Maria
> > > >
> > > >Maria A. Geraci
> > > >Pathology/Immunohistochemistry
> > > >Schering-Plough Research Institute
> > > >P.O. Box 32 Route 94 South
> > > >Lafayette, New Jersey 07848-0032
> > > >
> > > >Phone: (973) 940-4284
> > > >FAX: (973) 940-4183
> > > >maria.geraci@spcorp.com
> > > >
> > > >
> > > >
> > > >-----Original Message-----
> > > >From: Linda Stirpe [mailto:lpstirpe@yahoo.com]
> > > >Sent: Wednesday, July 02, 2003 7:51 PM
> > > >To: HistoNet Server
> > > >Subject: Humidity and Xylene Subs
> > > >
> > > >
> > > >Hi Fellow Histonetters,
> > > >
> > > >Now that summer and humidity is upon us our lab is
> > > >having the usual "bleeding eosin" problem. Last year
> > > >we were staining by hand but this year we were lucky
> > > >enough to get an automatic stainer which we thought
> > > >would take care of the problem - guess again. We are
> > > >using Richard Allan Clear Rite 3 which I know loves to
> > > >absorb water. I remember some literature about
> > > >"absorbant beads" to place in the Xylene Subs to help
> > > >with this problem. Since I cannot find the literature
> > > >- does anyone know the name, supplier, price, etc. and
> > > >most important do they work? Thanks!
> > > >
> > > >Have a great holiday!
> > > >
> > > >Linda Stirpe
> > > >Corning Hospital
> > > >Corning, NY 14830
> > > >
> > > >__________________________________
> > > >Do you Yahoo!?
> > > >SBC Yahoo! DSL - Now only $29.95 per month!
> > > >http://sbc.yahoo.com
> > > >
> > > >
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