RE: Humidity and Xylene Subs

From:Pamela Marcum

Kevin,
 
Drying the bags sounds like a good idea and would save money.  However, the oven had better be under a hood or the fumes will drive you out and are dangerous. 
 
Pam Marcum
-----Original Message-----
From: kevin williams [mailto:akwilliams75@hotmail.com]
Sent: Wednesday, July 09, 2003 2:35 PM
To: maria.geraci@spcorp.com; histonet@pathology.swmed.edu
Subject: RE: Humidity and Xylene Subs

Dear Maria:

I have just found some info on a company called Sun Cycle Technologies.

1866-786-2925

They appartently have some absorbant bags that you can put in the oven after use, dry out and re-use.

Sounds intresting I will be looking into both options.

Thanks for your help,

A.Kevin Williams 

>From: "Geraci, Maria"
>To: "'kevin williams'"
>Subject: RE: Humidity and Xylene Subs
>Date: Wed, 9 Jul 2003 11:32:57 -0400
>
>Hi Kevin,
>
>The company I order from is called: ACROS Organics. I believe the order
>number is 197245000. They are called: Molecular Sieves 3A, 4 to 8 mesh.
>
>Maria
>
>Dear Maria:
>
>Thanks for getting back to me I am having a lot of problems with the water.
>I though my reagent bottles may be contaminated, but this sounds tike the
>humidity. I was wondering if you could tell me the company name so I can do
>some investigating.
>
>Thanks for your help.
>
>Yours faithfully,
>
>A.Kevin Williams.
>
>
>
> >From: "Geraci, Maria"
> >To: "'kevin williams'"
> >Subject: RE: Humidity and Xylene Subs
> >Date: Mon, 7 Jul 2003 13:56:22 -0400
> >
> >Hi Kevin,
> >
> >I don't know if it will work on xylene substitutes, I've never tried. We
> >use recycled xylene in our lab and it works on the recycled xylene. The
>mesh
> >size is related to the chemical you are trying to dry out. I had to talk
> >with the manufacturer in order to know what size sieve to use. The sieves
> >are actual ceramic type beads that are placed at the bottom of your xylene
> >container and they absorb water to keep the xylene dry. They do not
> >actually come in contact with your slides. It is hard to know when they are
>
> >exhausted so when you start getting water droplets on your slides, you know
>
> >it's time to change the beads. Hopefully you'll do a test run each time you
>
> >process so you don't ruin your actual slides. These sieves can be used in
> >100% alcohol also to prevent water absorption. They work really well.
> >
> >Maria
> >
> >-----Original Message-----
> >From: kevin williams [mailto:akwilliams75@hotmail.com]
> >Sent: Monday, July 07, 2003 1:27 PM
> >To: maria.geraci@spcorp.com
> >Subject: RE: Humidity and Xylene Subs
> >
> >
> >This message was scanned by MIMEsweeper and determined to possibly be Spam
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> >
> >
> >
> >
> >
> >
> >Dear Maria:
> >
> >I am intrested to know if you can use this for Xylene sub, and it apears as
>
> >though it is a mesh that you place on the surface, is this correct? Do you
> >take it off when the slides are being processed? Or do I have totally the
> >wrong idea.
> >
> >Thanks for your help.
> >
> >Yours faithfully,
> >
> >A.Kevin Williams.
> >
> >
> >
> > >From: "Geraci, Maria"
> > >To: 'Linda Stirpe' , HistoNet Server
> > >Subject: RE: Humidity and Xylene Subs
> > >Date: Mon, 07 Jul 2003 09:21:05 -0400
> > >
> > >Hi Linda,
> > >
> > >We have the same problem in our laboratory in the summer and I use
> >Molecular
> > >Sieves 3A, 4-8 mesh put out by ACROS Organics. I believe the order number
>
> > >is 197245000. And yes, they really do work. I just add them to the xylene
>
> > >and I change them when I change the xylene.
> > >
> > >Good Luck
> > >
> > >Maria
> > >
> > >Maria A. Geraci
> > >Pathology/Immunohistochemistry
> > >Schering-Plough Research Institute
> > >P.O. Box 32 Route 94 South
> > >Lafayette, New Jersey 07848-0032
> > >
> > >Phone: (973) 940-4284
> > >FAX: (973) 940-4183
> > >maria.geraci@spcorp.com
> > >
> > >
> > >
> > >-----Original Message-----
> > >From: Linda Stirpe [mailto:lpstirpe@yahoo.com]
> > >Sent: Wednesday, July 02, 2003 7:51 PM
> > >To: HistoNet Server
> > >Subject: Humidity and Xylene Subs
> > >
> > >
> > >Hi Fellow Histonetters,
> > >
> > >Now that summer and humidity is upon us our lab is
> > >having the usual "bleeding eosin" problem. Last year
> > >we were staining by hand but this year we were lucky
> > >enough to get an automatic stainer which we thought
> > >would take care of the problem - guess again. We are
> > >using Richard Allan Clear Rite 3 which I know loves to
> > >absorb water. I remember some literature about
> > >"absorbant beads" to place in the Xylene Subs to help
> > >with this problem. Since I cannot find the literature
> > >- does anyone know the name, supplier, price, etc. and
> > >most important do they work? Thanks!
> > >
> > >Have a great holiday!
> > >
> > >Linda Stirpe
> > >Corning Hospital
> > >Corning, NY 14830
> > >
> > >__________________________________
> > >Do you Yahoo!?
> > >SBC Yahoo! DSL - Now only $29.95 per month!
> > >http://sbc.yahoo.com
> > >
> > >
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