RE: HIER and type II collagen

From:Mass Histology Service

Have you tried skipping the pressure cooker and covering the sections in
pronase for 10 minutes instead?  Try it, you might like it.

Jim

____________________________
  James E. Staruk, HT(ASCP)
   Mass Histology Service
(A division of DVMLab, Inc.)
   www.masshistology.com

-----Original Message-----
From: mrl0627@mail.ecu.edu [mailto:mrl0627@mail.ecu.edu]
Sent: Thursday, July 10, 2003 2:46 PM
To: histonet@pathology.swmed.edu
Subject: HIER and type II collagen



Hello all!

We have been using antibody against type II collagen in mouse tissue fixed
in Dent's and paraffin embedded successfully but have started using HIER
with pressure cooker and citrate buffer and this antibody doesn't seem to be
working (no staining in areas of limb cartilage that have always stained
before).  All other antibodies seem to love the AR (sections on same slide,
incubated with antibodies against neurofilament, against myosin, against
versican, all lit up beautifully).
Does anybody know of a particular problem with 11-116B3 or other antibodies
against type II collagen?
As always, TIA
Maureen Loomer
M.S. candidate (Cell Biology)
East Carolina University










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